Is Matter Around Us Pure
Chromatography is used for separation of those solutes which dissolve in same solvent 
Chromatography is a method of separating and identifying various components in a mixture, which are present in small trace quantities. 
Mixture containing two solid substances out of which one is soluble in a particular solvent and other is insoluble can be separated by dissolving the soluble constituent in a suitable solvent and then separating the insoluble substance through filtration. 
This process of separating the suspended particles of an insoluble substance, from a liquid, by rotating it at high speed is called centrifugation.  
The change in which the shape, size, appearance or state of a substance may alter but its chemical composition remains the same is called a physical change. In a physical change no new substance is formed. 
Any change that involves the formation of a new substance and leads to a transformation of chemical identity is called chemical change. 
Chemical changes are usually accompanied with heat exchanges. Chemical changes are permanent changes which are usually irreversible. 
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NCERT 9 Science